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  • Using PCR in real time for the detection of resistance genes problematic gram-negative bacteria

    Редакция | 2015, Practical medicine 04 (15) Innovative technologies in medicine. Part 2 | 15 июня, 2015

    M.R. MAZITOV, I.R. VALIULLINA, L.Yu. KULAGINA

    Republican Clinical Hospital of the MH of RT, 138 Orenburgskiy Tract, Kazan, Russian Federation, 420064 

    Mazitov M.R. — Chief Specialist for diagnosis, tel. (843) 237-31-58, е-mail: marat.mazitov@tatar.ru

    Valiullina I.R. — Head of the Laboratory of Clinical Bacteriology, tel. (843) 237-34-13, е-mail: innalife@yandex.ru

    Kulagina L.Yu. — Head of the Department of Clinical Pharmacology, tel. (843) 237-35-12, е-mail: kazanfarm@yandex.ru

    The growth of antimicrobial resistance in bacteria leads to a drastic reduction in the efficiency of causal treatment. Traditional methods of diagnosis in microbiology not prove the presence or absence of resistance genes. You must usually make greater use of modern methods for the determination of the genome of microorganisms. The article presents the results of studies of the genome 279 strains of gram-negative bacteria. This study should be carried out on a regular basis for monitoring of problem microorganisms and correction algorithms antibiotic therapy in the hospital.

    Key words: antibiotic resistance, molecular genetic microbiological monitoring.

     

     

    REFERENCES

    1. MUK 4.2.1890-04 opredelenie chuvstvitel’nosti mikroorganizmov k antibakterial’nym preparatam [MUK 4.2.1890-04 determining the sensitivity of microorganisms to antibiotics].
    2. Klinicheskie rekomendatsii. Opredelenie chuvstvitel’nosti mikroorganizmov k antibakterial’nym preparatam. Proekt. Versiya 2014-01 [Clinical practice guidelines. Determination of the sensitivity of microorganisms to antibiotics. Project. Version 2014-01].
    3. Molekulyarno-epidemiologicheskiy monitoring v sisteme epidemiologicheskogo nadzora za infektsiyami, svyazannymi s okazaniem meditsinskoy pomoshchi. Metodicheskie rekomendatsii [Molecular epidemiological monitoring system for epidemiological surveillance of infections associated with health care. Guidelines]. Moscow, 2014.
    4. Rukovodstvo po primeneniyu naborov reagentov dlya obnaruzheniya spetsificheskikh uchastkov DNK vozbuditeley infektsiy metodom PTsR s fluorestsentnoy detektsiey rezul’tata v rezhime real’nogo vremeni (RealTime) [Guidelines for the application kits for the detection of specific regions of DNA of pathogens by PCR with fluorescence detection results in real-time (RealTime)]. Format FLUOROPOL-RV. OOO NPF “Litekh”,2014.

    Метки: antibiotic resistance, I.R. VALIULLINA, L.Yu. KULAGINA, M.R. MAZITOV, molecular genetic microbiological monitoring, Practical medicine 04 (15) Innovative technologies in medicine. Part 2

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