Using PCR in real time for the detection of resistance genes problematic gram-negative bacteria
M.R. MAZITOV, I.R. VALIULLINA, L.Yu. KULAGINA
Republican Clinical Hospital of the MH of RT, 138 Orenburgskiy Tract, Kazan, Russian Federation, 420064
Mazitov M.R. — Chief Specialist for diagnosis, tel. (843) 237-31-58, е-mail: marat.mazitov@tatar.ru
Valiullina I.R. — Head of the Laboratory of Clinical Bacteriology, tel. (843) 237-34-13, е-mail: innalife@yandex.ru
Kulagina L.Yu. — Head of the Department of Clinical Pharmacology, tel. (843) 237-35-12, е-mail: kazanfarm@yandex.ru
The growth of antimicrobial resistance in bacteria leads to a drastic reduction in the efficiency of causal treatment. Traditional methods of diagnosis in microbiology not prove the presence or absence of resistance genes. You must usually make greater use of modern methods for the determination of the genome of microorganisms. The article presents the results of studies of the genome 279 strains of gram-negative bacteria. This study should be carried out on a regular basis for monitoring of problem microorganisms and correction algorithms antibiotic therapy in the hospital.
Key words: antibiotic resistance, molecular genetic microbiological monitoring.
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