E.M. MINGAZOVA¹, T.A. KHABELOVA¹, D.A. VALISHIN¹, Yu.V. VAKHITOVA², O.I. KUTUEV¹, L.R. SHAYKHULLINA¹

¹Bashkir Medical University, 3 Lenin St., Ufa, Russian Federation, 450000

²Institute of Biochemistry and Genetics, Ufa Scientific Center of RAS,  69 Oktyabrya Pr., Ufa, Russian Federation, 450054

Mingazova E.M. — postgraduate student of the Department of Infection Diseases with Course IAS, tel. (347) 250-59-58, e-mail: mingazovae@mail.ru

Khabelova T.A. — Cand. Med. Sc., Docent of the Department of Infection Diseases with Course IAS, tel. (347) 250-18-83, e-mail: thabelova@mail.ru

Valishin D.A. — D. Med. Sc, Professor of the Department of Infection Diseases with Course IAS, tel. (347) 250-18-83, e-mail: damirval@yandex.ru

Vakhitova Yu.V. — D. Biol. Sc., Head of the Laboratory of Molecular Pharmacology and Immunology, tel. (347) 235-60-88, e-mail: juvv73@gmail.com

Kutuev O.I. — Cand. Med. Sc., Docent of the Department of Infection Diseases with Course IAS, tel. (347) 250-18-99, e-mail: okutuev@yandex.ru

Shaykhullina L.R. — Cand. Med. Sc., Docent of the Department of Infection Diseases with Course IAS, tel. (347) 250-18-80, e-mail: liashaih@mail.ru

To investigate the association polymorphic variants in genes of cytokines with endotoxic shock, hemorrhagic syndrome, renal failure for patients with hemorrhagic fever with renal syndrome. The polymorphic regions -308G>A of the TNFA gene, -511C>T, 3953С>T of the IL1B gene, IL1RN intron 2 VNTR polymorphism, (CCTTT)_n polymorphism of the NOS2A gene were analysed by polymerase chain reaction.

The risk of developing endotoxic shock was increased of carriers allele TNFA*А (p=0,01; OR=2,13; 95%CI 1,15-3,91). Individuals carrying genotype IL1B*C/*T (-511C>T) and C/T-I/I (IL1В*-511C>T — IL1RN*VNTR) haplotype exhibited more than double risk of developing endotoxic shock (p=0,03; OR=2,78; 95%CI 1,10-7,38 and p=0,02: OR=2,67; 95%CI 1,15-6,13 respectively). Also, genotype NOS2A*14/*16 was higher in patients with endotoxic shock (p=0,04; OR=4,01; 95%CI 1,49-10,85). The relative risk (OR) of developing hemorrhagic syndrome for carriers T/T-C/T haplotype of IL1B (-511С>Т, 3953С>Т) was 6,4 (p=0,009; 95%CI 1,45-21,3). The allele NOS2A*11 (p=0,005; OR=2,85; 95%CI 1,28-6,25) and NOS2A*11/*14 genotype (p=0,005; OR=5,17; 95%CI 1,97-13,57) were increased in patients with renal failure.

Key words: polymorphism, hemorrhagic fever with renal syndrome, endotoxic shock, hemorrhagic syndrome, renal failure.

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