F.F. RIZNANOVA¹, O.I. PIKUZA², E.V. GENERALOVA², R.A. FAYZULLINA², O.A. KRAVTSOVA¹

¹Kazan (Volga) Federal University, 18 Kremlevskaya Str., Kazan, Russian Federation, 420008

²Kazan State Medical University of the Ministry of Health of the Russian Federation, 49 Butlerov Str., Kazan, Russian Federation, 420012

 Rizvanova F.F. — PhD (medicine), Associate Professor of the Department of Fundamental Principles of Clinical Medicine, e-mail: frizvanova@gmail.com, ORCID ID: 0000-0001-6320-8034

Pikuza O.I. — D. Sc. (medicine), Professor of the Department of Propaedeutics of Pediatric Diseases and Faculty Pediatrics, ORCID ID: 0000-0002-2885-5954

Generalova E.V. — PhD (medicine), Associate Professor of the Department of Propaedeutics of Pediatric Diseases and Faculty Pediatrics, e-mail: elena_generalova@rambler.ru, ORCID ID: 0000-0002-4577-2317

Faizullina R.A. — D. Sc. (medicine), Head of the Department of Propaedeutics of Pediatric Diseases and Faculty Pediatrics, e-mail: r868@mail.ru, ORCID ID: 0000-0002-7209-5737

Kravtsova O.A. — PhD (biology), Associate Professor of the Department of Biochemistry and Biotechnology, e-mail: okravz@yandex.ru, ORCID ID: 0000-0002-4227-0080

Objective: to identify the molecular genetic risk factors for the development of community-acquired pneumonia in children based on the analysis of polymorphism of pro- and anti-inflammatory interleukins.

Material and methods: A comprehensive clinical, laboratory and instrumental examination of 69 children aged 6 to 15 years with acute community-acquired pneumonia (CAP) was performed. Clinical, laboratory and instrumental examination of children was carried out according to generally accepted methods. Special research methods: the analysis of the relationship of polymorphic variants of cytokine genes il6 (-174) C/G and il4 (-590) C/T in patients with CAP and in the control group. DNA samples obtained from the peripheral venous blood of children and scraping epithelial cells from the oral mucosa served as the material for molecular genetic research.

Results: the incidence of acute community-acquired pneumonia is associated with polymorphism (-174) of the C/G gene il6. At the same time, the predisposing effect of the polymorphism (-590) C/T of the il4 gene to acute community-acquired pneumonia differs in different age groups.

Conclusion: The study also permitted an assessment of the significance of polymorphic markers of cytokine genes (il6 -174С/T and il4 -590С/T), regulating their synthesis, in the risk of occurrence and development of the main phenotypic manifestations of acute lung pathology in children.

Key words: children, community-acquired pneumonia, genetic polymorphism.

(For citation: Rizvanova F.F., Pikuza O.I., Generalova E.V., Faizullina R.A., Kravtsova O.A. Polymorphism of genes and community-acquired pneumonia in children. Practical Medicine. 2018)

REFERENCES

1. Zdravookhranenie v Rossii. 2017: stat. sb. Rosstat [Health care in Russia. 2017: stat. compilation. Rosstat]. Moscow, 2017. 170 p.
2. Tsarʹkova S.A., Kuznetsov P.V., Kupreeva N.G. Pneumonia in children: old problems and new opportunities. Pediatricheskaya farmakologiya, 2011, vol. 8, no. 1, pp. 12–16 (in Russ.).
3. Yatsyshina S.B., Spichak T.V., Kim S.S. et al. Novye vozmozhnosti v opredelenii ehtiologii vnebolʹnichnoy pnevmonii u detey [New opportunities in determining the etiology of community-acquired pneumonia in children]. Molekulyarnaya diagnostika: sb. trudov IХ Vseros. nauch.-praktich. konferentsii s mezhdunarodnym uchastiem. Moscow, 2017. Pp. 259–260.
4. Sabitova O.N., Sovalkin V.I. Gene polymorphism and production of major immunoregulatory cytokines in community-acquired pneumonia. Omskiy nauchnyy vestnik, 2009, no. 1 (84), pp. 42–45 (in Russ.).
5. Smirnova S.V., Salʹnikova L.E. The risk of pneumonia and polymorphism of TLR2 and TLR4 genes: a meta-analysis. Obshchaya reanimatologiya, 2015, vol. 11, no. 6, pp. 6–18 (in Russ.).
6. Balpanova G.T., Shortanbaev A.A., Tarabaeva A.S., Bitanova E.Zh. The role of cytokine gene polymorphism in the infectious process. Vestnik Natsionalʹnoy akademii nauk Respubliki Kazakhstan, 2013, no. 2, pp. 39–44 (in Russ.).
7. Patel J.A., Nair S., Revai K. Nasopharyngeal acute phase cytokines in viral upper respiratory infection. The Pediatric Infection Disease Journal, 2009, no. 11, pp. 1002–1007.
8. Pikuza O.I., Rizvanova F.F., Generalova E.V., Kravtsova O.A. Gene polymorphism of pro- and anti-inflammatory cytokines and acute bronchitis in children. Rossiyskiy vestnik perinatologii i pediatrii, 2017, vol. 62, no. 5, pp. 136–138 (in Russ.).