L.T. BAYAZITOVA¹, O.F. TUPKINA¹, T.A. CHAZOVA¹, Yu.A. TYURIN^1,2, I.D. RESHETNIKOVA¹, N.Yu. TYURINA⁴

¹Kazan Scientific-Research Institute of Epidemiology and Microbiology; 67 Bolshaya Krasnaya Str., Kazan, Russian Federation, 420015

²Kazan State Medical University, 49 Butlerov Str., Kazan, Russian Federation, 420012

³Kazan (Volga Region) Federal University, 18 Kremlyovskaya Str., Kazan, Russian Federation, 420008

⁴Stomatological Clinic №9, 43 Chistopolskaya Str., Kazan, Russian Federation, 420124 

Bayazitova L.T. ― Cand. Med. Sc., Head of the Scientific Research Laboratory of Microbiology, tel. (843) 236-67-91, e-mail: bajalt@mail.ru

Tyupkina O.F. ― Senior Researcher of the Scientific Research Laboratory of Microbiology, tel. (843) 236-67-91, e-mail: ant11@mail.ru

Chazova T.A. ― Junior Researcher of the Scientific Research Laboratory of Microbiology, tel. (843) 236-67-91, e-mail: kazkon@yandex.ru

Tyurin Yu.A. ― Cand. Med. Sc., Head of the Scientific Research Laboratory of Immunology and Allergens Development, Assistant of the Department of biological сhemistry, tel. (843) 236-67-91, e-mail: immunolab@mail.ru

Shamsutdinov A.F. ― Junior Researcher, tel. +7-909-306-72-72, e-mail: immunolab@yandex.ru

Reshetnikova I.D. ― Cand. Med. Sc., Deputy Director on Research work, tel. (843) 236-67-81, e-mail: reshira@mail.ru

Tyurina N.Yu. ― stomatologist, tel. (843) 523-44-88, e-mail: chistopolskaya@stomat9.ru 

The article presents the results of a study of antibiotic resistance of 191 methicillin-sensitive (MSSA) and methicillin-resistant (MRSA) strains of Staphylococcus aureus (S. aureus), taken from out-hospital and hospital patients. The data were received of molecular-genetic typing of S. aureus strains and types of SCCmec cassettes were established. There were significant differences in the sample genotypes of MRSA strains taken from out-hospital and hospital patients. The straines taken from out-hospital patients contained genetic elements SCCmec IV a, c, d types. The MRSA taken from hospital patients profile contained only type II SCCmec cassettes. Vancomycin, fusidic acid and mupirocin possess high activity against both MRSA and MSSA. MRSA were more resistant to antimicrobial drugs compared to MSSA. Azithromycin and clindamycin demonstrated moderate anti-staphylococcal activity. Low activity of chloramphenicol, tetracycline, erythromycin against staphylococci strains was revealed.

Key words: methicillin-resistant strains (MRSA), methicillin-sensitive strains (MSSA), Staphylococcus aureus, genetic types of SCCmec.

REFERENCES

1. Semina H.A., Kovaleva E.P., Akimkin V.G. Features of epidemiology and epidemiological surveillance of hospital infections at the present stage. Epidemiologiya i infektsionnye bolezni, 2006, no. 4, pp. 22-26 (in Russ.).
2. Tutel’yan A.V., Pisarev V.M., Gaponov A.M., Akimkin V.G. Prospects for research in the field of infection prevention research related to health care. Epidemiologiya i infektsionnye bolezni, 2010, no. 2, pp. 10-12 (in Russ.).
3. Chambers H. The Changing epidemiology of Staphylococcus aureus? Emerg. Inf. Dis, 2001, no. 7, pp. 178-182.
4. Palavecino E. Community-acquired methicillin-resistant Staphylococcus aureus infections. Clin. Lab. Med, 2004, no. 24, rr. 403-418.
5. Ito T., Kuwahara K., Hiramatsu K. Staphulococcal cassette chromosome mec (SScmec) analysis of MRSA. Methods Mol Biol, 2007, vol. 391, pp. 87-102.
6. Katayama J., Ito T., Hiramatsu K. A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother, 2000, vol. 44, pp. 1549-1555.
7. Fey P.D., Said-Salim B., Rupp M.E. et al. Comparative molecular analysis of community- or hospital-acquired methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother, 2003, vol. 47, pp. 96-203.
8. Okuma K., Iwakawa K., Turnidge J.D., et al. Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community. J. Clin. Microbiol, 2002, vol. 40, pp. 4289-4294.
9. Robinson D.A., Enright M.C. Evolutionary models of the emergence of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother, 2003, vol. 47, pp. 3926-3934.
10. Wielders C.L., Brisse S., de Graaf-Miltenburg L.A. et al. In-vivo transfer of mecA DNA to Staphylococcus aureus. Lancet, 2001, vol. 357, pp. 1674-1675.
11. Hiramatsu K., Longzhu C., Kuroda M., Ito T. The emergence and evolution of methicillin-resistant Staphylococcus aureus. Trend Microbiol, 2001, vol. 9, pp. 486-493.
12. Metitsillinrezistentnye Staphylococcus aureus ― vozbuditeli vnutribol’nichnykh infektsiy: identifikatsiya i genotipirovanie: Metodicheskie rekomendatsii [Methicillin-resistant Staphylococcus aureus — pathogens of nosocomial infections: identification and genotyping: Guidelines]. Moscow: Federal’nyy tsentr gigieny i epidemiologii Rospotrebnadzora, 2006. 43 p.