A.K. SHERSTENIKOVA, S.L. KASHUTIN, L.M. ZHURAVLEV, V.S. NEKLYUDOBA, E.I. TEDDER

Northern State Medical University, 51 Troitskiy prospekt, 163000 Arkhangelsk, Russian Federation

 Sherstennikova A.K. — Cand. Med. Sc., Associate Professor of the Normal Physiology Department, e-mail: a.sherstennikova@yandex.ru

Kashutin S.L. — Doc. Med. Sc., Head of the Skin and Venereal Diseases Department, e-mail: sergeycash@yandex.ru

Zhuravlev L.M. — resident of the Surgery Department, e-mail: leonidshagrov@mail.ru

Neklyudova V.S. — Assistant Lecturer of the Skin and Venereal Diseases Department, e-mail: viknek999@yandex.ru

Tedder E.I. — Assistant Lecturer of the Skin and Venereal Diseases Department, e-mail: tedder@mail.ru

(For citation: Sherstennikova A.K., Kashutin S.L., Zhuravlev L.M., Neklyudova V.S., Tedder E.I. Obtaining isolated cells of epidermis and derma by method of cellular alkaline dissociation. Practical Medicine. 2018)

Objective. To substantiate application of the method to obtain isolated cells of the dermis and epidermis for their further identification.

Materials and methods. The technique of cell-alkaline dissociation of skin cells in the modified version was carried out in accordance with the patent of the Russian Federation 2617237 “Method of obtaining preparations of isolated cells of the dermis” proposed by L.M. Zhuravlev et al.

Results. Using the comparative analysis of microscopic sections of psoriatic papules, keloid scar, slice of acne papules and the results of alkaline dissociation of corresponding bioptates, the possibilities of using cell-base dissociation in dermatology were shown.

Conclusions. The use of alkaline dissociation method allows obtaining information on the isolated cellular elements of the epidermis and dermis, including hematopoietic origin, degree of differentiation of keratinocytes based on their mitotic activity, which is not possible to achieve in the study of microscopic sections.

Key words: isolated cells, epidermis, dermis.

REFERENCES

1. Tsvetkova G.M., Mordovtseva V.V., Vavilov A.M., Mordovtsev V.N. Patomorfologiya bolezney kozhi: Rukovodstvo dlya vrachey [Pathomorphology of skin diseases: A guide for doctors]. Moscow: Meditsina, 2003. 496 p.
2. Volnukhin V.A., Vavilov A.M., Kravtsova I.V., Katunina O.R. Histological and immunohistochemical changes in the skin of patients with psoriasis in the treatment of PUVA baths. Vestnik dermatologii i venerologii, 2007, no. 2, pp. 3–7 (in Russ.).
3. Katunina O.R., Rezaykina A.V. Immunohistochemical and morphometric analysis of the content of Toll-like receptors 2 and 4, Langerhans cells and T-lymphocyte subpopulations in the affected skin of patients with psoriasis. Immunopatologiya, allergologiya, infektologiya, 2012, no. 2, pp. 51–54 (in Russ.).
4. Novikov A.I., Kononov A.V., Okhlopkov V.A., Pravdina O.V. Immunochemical study for psoriasis. Vestnik dermatologii i venerologii, 2003, no. 3, pp. 26–28 (in Russ.).
5. Novikov A.I., Okhlopkov V.A., Gubareva A.B. Morphological characteristics of the elements of rash in acne (biopsy study). Klinicheskaya dermatologiya i venerologiya, 2007, no. 2, pp. 62–67 (in Russ.).
6. Pavlov A.V., Shashkina M.V., Gansburgskiy M.A. et al. Obtaining isolated cells for cytological and cytogenetic studies of the epithelium of the thyroid gland. Morfologiya, 2006, vol. 130, no. 6, pp. 81–83 (in Russ.).
7. Belov L.N., Kogan M.E., Leontʹeva T.A. et al. Obtaining isolated cells by the method of alkaline dissociation of formalin-fixed tissues. Tsitologiya, 1975, no. 17(11), pp. 1332–1338 (in Russ.).
8. Zhuravlyov L.M., Vilova K.G., Mizgiryov D.V., Zashikhin A.L., Kashutin S.L. Sposob polucheniya preparatov izolirovannykh kletok dermy. Patent RF 2617237, zayavka 2015124695, 23.06.2015 [A method for preparing preparations of isolated cells of the dermis. The patent of the Russian Federation 2617237, application 2015124695, 06.23.2015].
9. Palʹtsev M.A., Ivanov A.A. Mezhkletochnye vzaimodeystviya [Intercellular interactions]. Moscow: Meditsina, 1995. 224 p.
10. Freydlin I.S., Totolyan A.A. Kletki immunnoy sistemy [Cells of the immune system]. Saint Petersburg: Nauka, 2001. 231 p.
11. Garifullina Eh.F., Khismatullina Z.R., Tukhvatullina Z.G. Impaired differentiation of epidermal cells in lymphoproliferative skin diseases. Prakticheskaya meditsina, 2012, no. 6 (61), pp. 106–109 (in Russ.).