A.S. BYKOV¹, S.E. BYKOV², N.V. BARYSHNIKOVA³, A.I GINAK¹

¹St. Petersburg State Technological Institute (technical university), 26 Moskovsky Prospect, Saint-Petersburg, Russian Federation, 190013

²Association of Medicine and Analytics, Co Ltd, 3 18-ya liniya V.O. St., St. Petersburg, Russian Federation, 199034

³I.P. PavlovFirst Saint-Petersburg State Medical University, 6-8 Lev Tostoy St., Saint-Petersburg, Russian Federation, 197022 

Bykov A.S. — Postgraduate Student of the Molecular Biotechnology Department, tel. +7-911-976-27-80, e-mail: bas1976@mail.ru¹

Bykov S.E. — Leading Specialist of Association of Medicine and Analytics, Co Ltd, tel. +7-921-400-22-78, e-mail: Bse_mail@mail.ru²

Baryshnikova N.V. — Cand. Med. Sc., Associate Professor of the Internal Diseases Department of Dental Faculty, tel. +7-921-301-33-77, e-mail: Baryshnikova_nv@mail.ru³

Ginak A.I. —D. Chem. Sc., Professor, Head of the Molecular Biotechnology Department, Academician of the RANS, tel. (812) 494-92-67, e-mail: mbt@lti-gti.ru¹

The article presents an arguable point concerning the transport of ammonia released in the stomach during urea hydrolysis in the presence of gastric urease to the mouth cavity. A model of such transport is suggested, based on physicochemical properties of NH3, current understanding of motility of the upper gastrointestinal tract as well as characteristics of «mouth cavity — esophagus — stomach» system air movement. Experimental findings obtained during direct rinsing of stomach walls with urea solution during gastroscopy showed that in spite of occurring losses ammonia released in the stomach can be easily detected in the mouth cavity air. The «oscillating» pattern of NH3 concentration in the mouth cavity over time is associated with rhythmic motor activity of the upper gastrointestinal tract. This understanding forms the basis of helicobacteriosis diagnosis by means of analysis of mouth cavity air composition.

Key words: helicobacteriosis diagnosis, ammonia transport, mouth cavity, diagnosis according to ammonia concentration.

REFERENCES

1. Kornienko E.A., Mileyko V.E. Gelik test – a method of noninvasive diagnosis of helikobakterioz. Ros. zhurnal gastroenter., gepatolog., koloproktol.,1998, no. 6, pp. 34–38 (in Russ.).

2. Parolova N.I. et al. Sravnitel’naya otsenka neinvazivnykh metodov diagnostiki Helicobacter Pylori u detey. Opyt primeneniya dykhatel’nogo Khelik-apparata. OOO “Assotsiatsiya meditsiny i analitiki” [Comparative assessment and other non-invasive methods of diagnosis Helicobacter Pylori in children. Experience of using breathing apparatus helika. «Association of Medicine and Analytics»], available at: http://www.amamed.ru/articles/article.php?action=43 (accessed: 03.07.2009).

3. Stepanov E.V. Diodnaya lazernaya spektroskopiya i analiz molekul-biomarkerov [Diode laser spectroscopy and molecular analysis of biomarkers]. Moscow, Fizmatlit, 2009. 416 p.

4. Nijevitch A.A., Yelicheva Z.M. Intragastric urea hydrolisis in children infected with Helicobacter pylori. J. Clin. Pathol, 1995, no. 48, p. 187.

5. Storonova O.A., Trukhmanov A.S. Metodika izucheniya dvigatel’noy funktsii pishchevoda, pod red. V.T. Ivashkina [Method for studying the motor function of the esophagus, edit. by V.T. Ivashkin]. Moscow, 2011. 36 p.

6. Kornienko E.A., Dmitrienko M.A., Klochko O.G., Nazhiganov O.N., Grigor’ev S.V., Mamaev N.A. Continuous registration of the ammonia concentration in the air in the diagnosis of oral infection Helicobacter pylori. Ros. zhurn. gastroenter., gepatol., koloproktol., 2003, vol. XIII, no. 5, appl. 21, materials of the 9 Gastronedeli, 2003, October, p. 159 (in Russ.).

7. Kolesnikov L.L. Sfinkterologiya [Sfinkterology]. Moscow: GEOTAR-media, 2008. 152 p.

8. Uspenskiy Yu.P., Suvorov A.N., Baryshnikova N.V. Infektsiya Helicobacter pylori v klinicheskoy praktike (monografiya) [Helicobacter pylori infection in clinical practice (monograph)]. Saint Petersburg: InformMed, 2011. 572 p.

9. Korot’ko G.V. Zheludochnoe pishchevarenie [Gastric digestion]. Krasnodar, 2007.

10. Baytinger V.F. Verkhniy pishchevodnyy sfinkter: strukturnye, funktsional’nye i klinicheskie aspekty [Upper esophageal sphincter: structural, functional and clinical aspects].