M.E. ZHELEZOVA¹, B.K. BEKTUR², L.I. MALTSEVA³, T.V. GRIGORYEVA⁴, E.A. BOULYGINA⁴, D.R. KHUSNUTDINOVA⁴, E.A. ZIATDINOVA¹

 ¹Kazan Federal University, Kazan

²Assisted Reproductive Technologies Ltd., Kazan

³Kazan State Medical Academy — Branch Campus of RMACPE MH Russia, Kazan

⁴Omics Technologies Research Laboratory, Institute of Fundamental Medicine and Biology of KFU, Kazan

 Contact details:

Zhelezova M.E. — MD, Professor of the Department of Surgery, Obstetrics and Gynecology

Address: 74 K. Marks St., Kazan, Russian Federation, 420012, tel.: +7 (843) 236-34-51, e-mail: gelezovam@gmail.com

The purpose — to assess the possible influence of the microbiome of the vagina, oral cavity and placenta of women on the late fetal growth retardation syndrome.

Material and methods. A prospective-retrospective analysis of pregnancy and childbirth in 80 pregnant women was carried out. The main group consisted of 40 women with late fetal growth retardation syndrome, the comparison group — 40 healthy pregnant women. The study of the microbiome of the vagina, oral cavity, and placenta was carried out by sequencing of nitrogenous bases in the 16S ribosomal RNA (r-RNK) genes in 15 patients with fetal growth retardation and 8 healthy pregnant women. The Fast-DNA SPIN Kit was used to isolate the DNA material. The isolated DNA was subjected to subsequent PCR amplification followed by sequencing with forward primers and reagent kits.

Results. The vaginal microbiome in women of both observation groups was characterized by the predominance of Lactobacillus, the content of which was more than 90%. The oral microbiome in both observation groups was marked by the predominance of two main bacterial types — Firmicutes and Prevotella, while Streptococcus was dominant in women with fetal growth retardation. In the placenta of women with fetal growth retardation, the bacterial diversity was significantly lower than in healthy pregnant women. The oral and placental microbiome influences the development of the late fetal growth retardation syndrome.

Conclusion. The influence of the altered maternal microbiome on the formation of late fetal growth retardation is not excluded.

Key words: vaginal microbiome, oral microbiome, placental microbiome, late fetal growth retardation syndrome.

REFERENCES

1. Colella M., Frerot A., Novais A.R.B., Baud O. Neonatal and Long-Term Consequences of Fetal Growth Restriction. Curr Pediatr Rev, 2018, vol. 14 (4), pp. 212–218.
2. Dunlop Anne L., Mulle Jennifer G., Ferranti Erin P. et al. Maternal microbiome and pregnancy outcomes that impact infant health. Advances in neonatal care, 2015, vol. 15 (6), pp. 377–385.
3. Hamza A., Herr D., Solomayer E.F., Meyberg-Solomayer G. Polyhydroamnios: causes, diagnosis and therapy. Geburtshilfe Frauenheilkd, 2013, vol. 73 (12), pp. 1241–1246.
4. Kyono K., Hashimoto T., Nagai Y., Sakuraba Y. Analysis of endometrial microbiota by 16S ribosomal RNA gene sequencing among infertile patients: a single-center pilot study. Reprod Med Biol, 2018, vol. 17 (3), pp. 297–306.
5. Abranches J., Zeng L., Kajfasz J.K., Palmer S.R., Chakraborty B., Wen Z.T. et al. Biology of oral streptococci. Microbiol Spectr, 2018, vol. 6 (5), p. 10.1128.
6. Koren O., Goodrich J.K., Cullender T.C, Spor A., Laitinen K., Bäckhed H.K. et al. Host remodeling of the gut microbiome and metabolic changes during pregnancy. Cell, 2012, vol. 150 (3), pp. 470–480.
7. Lin C.Y., Yeh Y.M., Yang L.Y., Lee Y.S., Chao A., Chin C.Y. et al. Severe preeclampsia is associated with a higher relative abundance of Prevotella bivia in the vaginal microbiota. Sci Rep, 2020, vol. 10 (1), pp. 18249–18254.
8. Shlomi Barak, Orit Oettiger-Barak, Eli E. Machtei, Hannah Sprecher, Gonen Ohel. Evidence of periopathogenic microorganisms in placentas of women with preeclampsia. Journal of periodontology, 2020, vol. 78 (4), pp. 670–676.
9. Crusell M.K.W., Hansen T.H., Nielsen T., Allin K.H., Ruhlemann M.C., Damm P. et al. Gestational diabetes is associated with change in the qut microbiota composition third trimester of pregnancy and postpartum. Microbiom, 2018, vol. 6 (1), pp. 89–94.
10. Blostein F., Gelaye B., Sanchez S.E., Williams M.A., Foxman B. Vaginal microbime diversity and preterm birth: results of a nested case-control study in Peru. Ann Epidemiol, 2020, vol. 41, pp. 28–34.
11. Aagaard K., Ma J., Antony K.M. et al. The placenta harbors a unique microbiome. Sci Transl Med, 2014, vol. 6, pp. 237–245.
12. Gomez-arango LF., Barett HL., Mclntyre HD. et al. Contributios of the maternal oral and gut microbiome to placental microbial colonization in overweight and obese pregnant women. Sci Rep, 2017, vol. 7 (1), pp. 2860–2868.
13. Zheng J., Xiao X., Zhang Q., Mao L., Yu M., Xu J. The placental microbiome varies in association with low birth weight in full-term neonates. Nutrients, 2015, vol. 7 (8), pp. 6924–6937.
14. Chen C., Tang P., Liang J. et al. Association between intrauterine microbiome and risk of intrauterine growth restriction: A case-control study based on Guangxi Zhuang birth cogort in China. Tohoku Journal of Experimental Medicine, 2022, pp. 2–41.
15. Gorkiewicz G., Moschen A., Gut microbiome: a new player in gastrointestinal disease. Virchows Arch, 2018, vol. 472 (1), pp. 159–172.