A.V. KOREL, A.А. VOROPAEVA, L.A. CHERDANTSEVA, I.A. KIRILOVA

Novosibirsk Research Institute of Traumatology and Orthopaedics named after Ya. L. Tsivyan

 Contact details:

Korel A.V. – Cand. Biol. Sc., Leading Researcher

Address: 1 Frunze St., Novosibirsk, Russian Federation, 630091, e-mail: AKorel@niito.ru

The effect was studied of the properties of demineralized bone matrix of spongy and compact human bone upon the properties of osteogenic and chondrogenic cells, which were populated on the bone matrix in order to create tissue engineering structures for regenerative medicine. The samples were preliminarily studied for physical-mechanical, strength, morphological characteristics and piezoelectric properties of demineralized bone matrix. In vitro, the possibility of populating the selected samples of demineralized bone matrix by osteoblast and chondrocyte cells and their effect on the matrix itself was determined. By cytochemical coloring method, the histological belonging of cells populated in the bone matrix was determined. The relationship was found between the evenness and depth of populating the demineralized bone matrix by osteogenic and chondrogenic cells and the processing conditions of the matrix, and matrix properties. The expressed resorptive ability of osteogenic cells in relation to the matrix material was revealed compared to the cells of chondrogenic series.

Key words: bone-plastic materials, tissue-engineering constructions, demineralized bone matrix, cultivation of cells, cells of osteogenic and chondrogenic series.

REFERENCES

1. Kirilova I.A. Anatomo-funktsional’nye svoystva kosti kak osnova sozdaniya kostno-plasticheskikh materialov dlya travmatologii i ortopedii: avtoref. dis. … d-ra med. nauk [Anatomical and functional properties of the bone as the basis for the creation of osteoplastic materials for traumatology and orthopedics. Synopsis of dis. Dr med. sciences]. Novosibirsk, 2011.
2. Nigmatullin R.T., Shcherbakov D.A., Musina L.M. et al. Some aspects of the clinical use of bone and cartilage allografts. Meditsinskiy vestnik Bashkortostana, 2012, vol. 7, no. 4, pp. 78–83 (in Russ.).
3. Shvets A.I., Ivchenko V.K. Bone grafts and their substitutes in spinal surgery. Ortopediya, travmatologiya i protezirovanie, 2008, vol. 3, pp. 66–69 (in Russ.).
4. Podorozhnaya V.T., Kirilova I.A., Sharkeev Yu.P. et al. The study of the structural and functional characteristics of the middle cuts of the heads of the femurs. Uspekhi sovremennogo estestvoznaniya, 2015, vol. 9, pp. 126–129 (in Russ.).
5. Grudyanov A.I., Nikolaev A.V. Comparative analysis of the long-term results of using various auto- and allografts to create a zone of keratinized gums in the lower jaw of vestibuloplasty. Stomatologiya, 2016, vol. 95, no. 1, pp. 40–43 (in Russ.).
6. Murphy S.V., Atala A. Organ engineering – combining stem cells, biomaterials, and bioreactors to produce bioengineered organs for transplantation. Bio essays, 2012, no. 35, pp. 163–172.
7. Kirilova I.A., Podorozhnaya V.T., Sharkeev Yu.P. et al. Properties of demineralized bone matrix for tissue bioengineering. Kompleksnye problemy serdechno-sosudistykh zabolevaniy, 2017, vol. 6, no. 3, pp. 25–36 (in Russ.).
8. Shchelkunova E.I., Voropaeva A.A., Korel’ A.V. et al. Population of the demineralized bone matrix by chondrogenic cells. Kompleksnye problemy serdechno-sosudistykh zabolevaniy, 2018, vol. 7, no. 2, pp. 102–111 (in Russ.).
9. Shvets A.I., Ivchenko V.K. Bone grafts and their substitutes in spinal surgery. Ortopediya, travmatologiya i protezirovanie, 2008, no. 3, pp. 66–69.
10. Hofer S., Leopold S.S., Jacobs J. Clinical perspectives on the use of bone graft based on allografts In: Laurencin CT, editor. Bone graft substitutes. West Conshohocken, PA: ASTM International, 2003, pp. 68–95.