I.V. NIKOLAEVA¹, G.S. SHAIKHIEVA², T.V. GRIGORYEVA³, I.U. VASILYEV³, E.S. GERASIMOVA⁴, N.S. LEONTIEVA⁴

 ¹Kazan (Volga) State Medical University, Kazan

²Republican Clinical Infectious Hospital named after Prof. A.F. Agafonov, Kazan

³ Kazan Federal University, Kazan

⁴CITILAB Association of Clinical Diagnostic Laboratories, Kazan

 Contact details:

Nikolaeva I.V. — MD, Professor, Head of the Department of Infectious Diseases

Address: 49 Butlerov St., Russian Federation, Kazan, 420012, tel.: + 7-960-037-70-17, e-mail: Irinanicolaeva @ mail.ru

Antibiotic resistance and resistome of intestinal strains of Kl. pneumoniae colonizing healthy babies in maternity hospitals were studied. According to the results of phenotype studies, Kl. pneumoniae in 92,3% of cases were ESBL producers and showed resistance to ampicillin, amoxiclav, co-trimoxazole, less to ciprofloxacin and nitrofurantoin. 90% of intestinal Kl. pneumoniae had multiple resistance to antimicrobial drugs. According to a genome-wide study, beta-lactamase genes were found in all strains. blaOXA-1 was found in 60%, blaTEM-30 in 70%, blaCTX-M-15 in 70%, blaSHV in 100% of the strains. Genes for resistance to aminoglycosides were found in 90%, fluoroquinolones — in 40%, sulfonamides — in 80%, chloramphenicol — in 70%, tetracycline — in 50%, and trimethoprim – in 70% of Kl. pneumoniae strains.

Key words: Klebsiella infection, antibiotic sensitivity, newborns, ESBL.

REFERENCES

1. Hampton T. Novel Programs and Discoveries Aim to Combat Antibiotic Resistance. JAMA, 2015, vol. 313 (24), pp. 2411–2413.
2. Borghesi A., Stronati M. Superbugs and antibiotics in the newborn. J Pediatr Neonat Individual Med, 2015, vol. 4 (2), r. e040253.
3. Jacquot A., Neveu D., Aujoulat F. et al. Dynamics and clinical evolution of bacterial gut microflora in extremely premature patients. J Pediatr, 2011, vol. 158 (3), rr. 390–396.
4. Glumcher F.S. Multidrug-resistant infection: relevance, definition, mechanisms, most common pathogens, treatment, prevention. Nauka i praktika, 2014, no. 1 (2), pp. 129–149 (in Russ.).
5. Endimiani A., Hujer K.M., Hujer A.M. et al. Evaluation of ceftazidime and NXL104 in two murine models of infection due to KPC-producing Klebsiella pneumoniae. Antimicrob. Agents Chemother, 2011, vol. 55 (1), pp. 82–85.
6. Temkin E., Fallach N., Almagor J. et al. Estimating the number of infections caused by antibiotic-resistant Escherichia coli and Klebsiella pneumoniae in 2014: a modelling study. Lancet Glob Health, 2018, vol. 6 (9), pp. e969–e979.
7. Khaertynov K., Anokhin V., Davidyuk Y. et al. Case of Meningitis in a Neonate Caused by an Extended-Spectrum Beta-Lactamase-Producing Strain of Hypervirulent Klebsiella pneumonia. Front. Microbiol, 2017, vol. 8 (1576), rr. 1–6.
8. Haller S., Eller C., Hermes J. et al. What caused the outbreak of ESBL-producing Klebsiella pneumoniae in a neonatal intensive care unit, Germany 2009 to 2012? Reconstucting transmission with epidemiological analysis and whole-genome sequencing. BMJ, 2015, vol. 5 (5), p. e007397.
9. Martin M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet.journal, 2011,vol. 17 (1), pp. 10–12.
10. Bolger A.M., Lohse M., Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics, 2014, vol. 30 (15), pp. 2114–2120.
11. Bankevich A., Nurk S., Antipov D. et al. SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing. J Comput. Biol, 2012, Vol. 19 (5), pp. 455–477.
12. Lam M.M., Wick R.R.​, Wyres K.L. et al. Genetic diversity, mobilisation and spread of the yersiniabactin-encoding mobile element ICEKp in Klebsiella pneumoniae populations. Microbial Genomics, 2018, vol. 4 (9), pp. 1–14.
13. Kozlova N.S., Barantsevich N.E., Barantsevich E.P. Antibiotic sensitivity of Klebsiella pneumoniae strains isolated in a multidisciplinary hospital. Infektsiya i immunitet, 2018, vol. 8, no. 1, pp. 79–84 (in Russ.).
14. Broberg C.A., Palacios M., Miller V.L. Klebsiella: a long way to go towards understanding this enigmatic jet-setter. F1000 Prime Reports, 2014, vol. 6 (64), rr. 1–12.
15. Tsaregorodtsev A.D., Khaertynov Kh.S., Anokhin V.A. et al. Klebsiella neonatal sepsis. Rossiyskiy vestnik perinatologii i pediatrii, 2016, no. 4, pp. 49–54 (in Russ.).
16. Moradigaravand D., Martin V., Peacock S. J. et al. Evolution and Epidemiology of Multidrug-Resistant Klebsiella pneumoniae in the United Kingdom and Ireland. MBio, 2017, vol. 21 (8), pp. 1–11.
17. Hendrik T.C., Voor in ‘t holt A.F., Vos M.C. Clinical and Molecular Epidemiology of Extended-Spectrum Beta-Lactamase-Producing Klebsiella spp.: A Systematic Review and MetaAnalyses. PLoS ONE, 2015, vol. 10 (10), pp e0140754.
18. Naas T., Cuzon G., Robinson A.L. et al. Neonatal infections with multidrug-resistant ESBL-producing E. cloacae and K. pneumoniae in neonatal units of two different hospitals in Antananarivo. Madagascar. BMC Infect. Dis, 2016, no. 16, r. 275.
19. Strachunskiy L.S. Extended-spectrum β-lactamase is a rapidly growing and poorly understood threat. Klin. mikrobiol. antimikrob. khimioter, 2005, vol. 7, no. 1, pp. 92–96 (in Russ.).
20. Huerta-García GC., Miranda-Novales G., Díaz-Ramos R. et al. Intestinal colonization by extended-spectrum beta-lactamase-producing enterobacteriaceae in infants, 2015, no. 67, rr. 313–320.