L.А. МUSINA, R.F. SHAKIROV, V.U. GALIMOVA, О.R. SHANGINA, A.I. LEBEDEVA, R.Z. КADIROV

«Russian Eye and Plastic Surgery Center» of the Russian Federation Health Ministry Ufa, Russian Federation

 Contact information:

Musina L.A. – D.Sc. (biology), Leading Researcher at the Morphology Department

Address: 67/1 R. Sorge St., Ufa, the Republic of Bashkortostan, Russian Federation, 450075, tel.: +7 (347) 293-42-35, e-mail: morphoplant@mail.ru

 The relevance of the research is stipulated by the dominant role of cytokines in the regulation of tissue regeneration processes in damages. Cytokines trigger the processes of tissue epithelization and granulation having a significant impact on scar formation.

Purpose. To determine the cytokoine profile during the regeneration process in the damaged cornea after circumlimbal insertion of the allogenic biomaterial.

Material and methods. Histological, immunohistochemical, morphometric and statistic methods were used to study the enucleated eyeballs of experimental rabbits after chemical burn by alkali (2.5% solution of sodium hydroxide) followed by a circumlimbal insertion of «Alloplant» biomaterial (dispersed form of allogenic biomaterial «Stimulator of regeneration»). The studies of the cornea and the eye limbal zone of the experimental animals were carried out on the 7^th, 14^th, 30^th, 90^th and 180^th days after the operation.

Results. It was established that after the perilimbal insertion of the Alloplant biomaterial into the damaged cornea of the experimental rabbits compared with the control group (with no treatment) the proliferative activity of the corneal epithelial cells increased, large amounts of phagocytic macrophages (CD68+cells) were involved, and the low level of cytokine expression was detected that transforms the growth factor TGF-β1 (fibrosis factor). These factors leads to the fast epithelization, accelerated utilization of the cell and tissue detritus, and inhibition of the gross scarring process of the restoring corneal tissues.

Conclusion. Circumlimbal insertion of «Alloplant» biomaterial into the damaged cornea serves as a regeneration stimulator and an inhibitor of tissue scarring.

Key words: «Alloplant» biomaterial, circumlimbal insertion, corneal regeneration, TGF-β1 transforming growth factor, scarring inhibitor.

REFERENCES

1. Galimova V.U., Shakirov R.F. Experience in the treatment of keratoconus using Alloplant transplantation technology. Kataraktal’naya i refraktsionnaya khirurgiya: nauchno-prakticheskiy zhurnal, 2012, vol. 12, no. 1, pp. 27–30 (in Russ.).
2. Galimova V.U., Shakirov R.F., Gareev E.M. The results of treating patients with keratoconus dispersed to varying degrees by the Alloplant biomaterial. Oftal’mologicheskie vedomosti, 2013, vol. 7, no. 1, pp. 26–28 (in Russ.).
3. Musina L.A., Shakirov R.F., Kadyrov R.Z., Shangina O.R. Experimental-Morphological Study of the Impact of Dispersive Allogenic Biological Material on the Regeneration of the Warrior. Prakticheskaya meditsina, 2018, vol. 16, no. 4, pp. 133–139 (in Russ.).
4. Lebedeva A.I., Muslimov S.A., Musina L.A. Experimental modeling of the process of chronic inflammation and fibrosis. Biomeditsina, 2013, no. 4, pp. 114–123 (in Russ.).
5. Obenberger J. Paper strips and rings as simple tools for standartization of experimental eye injuries. Ophthalmol. Res, 1975, vol. 7, pp. 363–366.
6. Khollender M., Vul’f D. Neparametricheskie metody statistiki [Non-parametric methods of statistics]. Moscow: Finansy i statistika, 1983. 518 p.
7. Rebrova O.Yu. Statisticheskiy analiz meditsinskikh dannykh. Primenenie paketa prikladnykh programm STATISTICA [Statistical analysis of medical data. Application software package STATISTICA]. Moscow: MediaSfera, 2002. 312 p.
8. Bagrov S.N. Sources of regeneration of the cornea. Oftal’mol. zhurn, 1980, no. 1, pp. 231–233 (in Russ.).
9. Gundorova R.A., Khoroshilova-Maslova I.P., Chentsova E.V., Ilatovskaya L.V., Yamskova V.P., Romanova I.Yu. Application of agon in the treatment of penetrating wounds of the cornea in the experiment. Vest. oftal’mol, 1997, vol. 113, no. 2, pp. 12–16 (in Russ.).
10. Muslimov S.A. Morfologicheskie aspekty regenerativnoy khirurgii [Morphological aspects of regenerative surgery]. Ufa.: Bashkortostan, 2000. 168 p.
11. Rodemann H.P., Binder A., Burger A., Guven N., Loffler H., Bamberg M. The underlying cellular mechanism of fibrosis. Kidney Int Suppl, 1996, vol. 54, pp. 32–36.
12. Zubova S.G., Danilov A.O., Okulov V.B. et al. Synthesis and expression of transforming growth factor-beta activated macrophages. Voprosy onkologii, 1996, vol. 42, no. 5, pp. 80–85 (in Russ.).
13. Muldashev E.R., Muslimov S.A., Musina L.A., Nigmatullin R.T., Lebedeva A.I., Shangina O.R., Khasanov R.A. The role of macrophages in the tissues regeneration stimulated by the biomaterials. Cell Tissue Bank, 2005, vol. 6, no. 2, pp. 99–107.