E.R. MULDASHEV¹, A.I. LEBEDEVA¹, S.A. MUSLIMOV¹, S.V. POPOV², S.A. AFANASYEV², D.S. KONDRATIEVA²

¹Russian Eye and Plastic Surgery Center, Ufa, the Republic of Bashkortostan, Russian Federation

²Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russian Federation

Contact details:

Muldashev E.R. – D.Sc. (medicine), Professor, President of “Russian Eye and Plastic Surgery Center” of the Ministry of Health of the Russian Federation

Address: 67/1 Richard Sorge St., Ufa, the Republic of Bashkortostan, Russian Federation, 450075, tel.: 8 (347) 224-68-01, e-mail: center@alloplant.ru

Stimulation of the cell regenerative potential of an adult myocardium is not adequately reflected in practical medicine. The proposed methods of cell engineering allow to achieve only paracrine effect from stem cells, but not their integration into the damaged myocardium. This slightly improves the structure of the ischemically damaged myocardium and is often associated with complications.

Objective: to identify the cellular potential of the myocardium by using dispersed allogeneic biomaterial (DAB), because Alloplant’s biomaterial is known to improve the structure of the ischemically damaged myocardium.

Material and methods. Male Wistar rats were used to ligate the coronary artery. In the experimental group, simultaneous ligation of the artery was accompanied by intramyocardial injection of DAB suspension into the stenotic artery pool, and in the control group physiological saline was injected. Histological, immunohistochemical, and electron microscopic methods of research were used.

Results. In terms of the use of dispersed allogenic biomaterial, a significant reduction in the scar area in the ischemic damaged myocardium was revealed. This study demonstrates stem cells and their transdifferentiation into mature cardiomyocytes with the restoration of cell-cell interactions. Research clearly shows their likely role in cell regeneration, and not through the paracrine effect.

 Key words: myocardium, ischemia, allogeneic biomaterial, stem cells.

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