Research into the mineral component and organic matrix of bone tissue using the method of atomic-force microscopy
A.S. KORSHUNOV1, S.N. MOSKOVSKIY1, V.P. KONEV1, I.L. SHESTEL1, D.O. SEROV1, Yu.O. SHISHKINA1, A.V. SUBOCH2, S.O. MARKOVSKiY3
1Omsk State Medical University, 12 Lenin Str., Omsk, Russian Federation, 644099
2Bureau of Forensic Medical Examination, 9 Pereleta Str., Omsk, Russian Federation, 644112
3City Clinical Hospital No. 1, 55 Nakhimov Str., Omsk, Russian Federation, 644105
Contact:
Korshunov A.S. — Assistant Lecturer of the Department of Maxillofacial Surgery, tel. +7-904-32-95-449, E-mail: Andrey_K_180588@mail.ru
Moskovskiy S.N. — Cand. Med. Sc., Associate Professor of the Department of Forensic Medicine and Law, tel. +7-960-997-63-13, e-mail: moscow55@mail.ru
Konev V.P. — Professor, D. Med. Sc., Head of the Department of Forensic
Medicine and Law, tel. +7-913-963-05-97, e-mail: vpkonev@mail.ru
Shestel I.L. — Cand. Med. Sc., Associate Professor of the Department of Forensic Medicine and Law, tel. +7-913-963-05-97, E-mail: vpkonev@mail.ru
Serov D.O. — clinical resident doctor of the Department of Maxillofacial Surgery,
tel. +7-913-148-31-68, e-mail: dimon-.93@mail.ru
Shishkina Yu.O. — Assistant Lecturer of the Department of Forensic Medicine and Law, tel. +7-913-963-05-97, E-mail: vpkonev@mail.ru
Suboch A.V. — Deputy Director of the Bureau of Forensic Medical Examination, tel. +7-913-96305-97, e-mail: vpkonev@mail.ru
Markovskiy S.O. — Head of the Department of Maxillofacial Surgery, tel. (3812) 28-42-62, e-mail: muzgkb11@mail.ru
The method of atomic-force microscopy of bone tissue allows studying the organic matrix and mineral component in detail. The method makes it possible to study the architectonics of the collagen fibers mineralization level. According to the results of the bone tissue investigation in the presence of connective tissue pathology using this method, it was possible to visualize the presence of cavities, which entails the change in the deposition structure of the bone mineral elements, the change in the formation of crystal combinations, and the change in the amount of mineral components per bone volume unit.
Key words: atomic-force microscopy, bone tissue.
REFERENCES
- Moskovskiy S.N., Korshunov A.S., Shestel’ I.L. Use of atomic force microscopy in the study of dense tissues of the orofacial region. Kazanskiy meditsinskiy zhurnal, vol. 93, no. 6, 2012, pp. 887–890 (in Russ.).
- Jaschouz D., Paris O., Roschger P., Hwang H.S., Fratzl P. Pole figure analysis of mineral nanoparticle orientation in individual trabecular of human vertebral bone. J. Appl. Crystallogr, 2003, vol. 36, pp. 494–498.
- Kuangshin T., Hang J.Q., Ortis C. Effect of mineral content on the nanoindentation properties and nanoscale deformation mechanisms of bovine tibial cortical bone. J. Materials science: Materials in medicine, 2005, vol. 16, no. 8, pp. 1–12.
- Landis W.J., Song M.J., Leith A., McEwen L. Mineral and organic matrix interaction in normally calcifying tendon visualized in three dimensions by high-voltage electron microscopic tomography and graphic image reconstruction. J. Struct. Biol, 1993, no. 110, pp. 39–54.
- Landis W.J., Hodgens K.J. Mineralization of collagen may occur on fibril surfaces: evidence from conventional and high-voltage electron microscopy and three-dimensional imaging. J. Struct. Viol, 1996, vol. 117, pp. 24–35.
- Lees S., Prostak K.S., Ingle V.K., Kjoller K. The loci of mineral in turkey leg tendon as seen by atomic force microscope and electron microscopy. Caicif. Tissue Int, 1994, vol. 55, pp. 180–189.
- Rubin M.A., Jasiuk L., Taylor J, Rubin J., Ganey T., Apkarian R.P. TEM analysis of the nanostructure of normal and osteoporotic human trabecular bone. Vone, 2003, vol. 33, no. 3, rr. 270–282.