A.Z. ZARIPOVA^1,2, L.T. BAYAZITOVA^1,2, O.F. TUPKINA¹, T.A. CHAZOVA¹, Y.A. TYURIN^1,2, G.Sh. ISAEVA^1,2, E.M. POKROVSKAYA³

 ¹Kazan Scientific and Research Institute for Epidemiology and Microbiology of Rospotrebnadzor, 67 Bolshaya Krasnaya Str., Kazan, Russian Federation, 420015

²Kazan State Medical University of the Ministry of Health of the Russian Federation, 49 Butlerov Str., Kazan, Russian Federation, 420012

³Kazan (Volga Region) Federal University, 18 Kremlevskaya Str., Kazan, Russian Federation, 420008

Zaripova A.Z. — Junior Research Associate of Scientific and Research Laboratory of Microbiology, tel. +7 (843) 236-67-91, e-mail: [email protected], ORCID ID: 0000-0001-6790-0538

Bayazitova L.T. — Ph. D. (medicine), Head of Scientific and Research Laboratory of Microbiology, associate Professor of the Department of Microbiology, tel. +7 (843) 236-67-91, e-mail: [email protected], ORCID ID: 0000-0002-2142-7682

Tyupkina O.F. — Senior Research Associate of Scientific and Research Laboratory of Microbiology, tel. +7 (843) 236-67-91, e-mail: [email protected], ORCID ID: 0000-0001-8180-1165

Chazova T.A. — Junior Research Associate of Scientific and Research Laboratory of Microbiology, tel. +7 (843) 236-67-91, e-mail: [email protected], ORCID ID: 0000-0002-2013-4239

Tyurin Y.A. — Ph. D. (medicine), Head of Scientific and Research Laboratory of Immunology and Allergen Development, tel. +7 (843) 236-67-91, e-mail: [email protected], ORCID ID: 0000-0002-2536-3604

Isaeva G.Sh. — D. Sc. (medicine), Director, Head of the Department of Microbiology, tel. +7 (843) 236-67-21, e-mail: [email protected], ORCID ID: 0000-0002-9719-9583

Pokrovskaya E.M. — Ph. D. (medicine), Assistant of the Department of Surgery of the Institute of Fundamental Medicine and Biology, tel. +7-917-263-90-96, е-mail: [email protected], ORCID ID: 0000-0001-9437-4895

 To assess the frequency of the prevalence of pneumococcal carriage in preschool children; to determine the serotype composition and to study the antibiotic sensitivity of pneumococci.

Material and methods. Phenotypic identification of S. pneumoniae was performed on the basis of morphological, cultural data. Species identification of cultures was confirmed by MALDI-TOF mass spectrometry. Testing of antibiotic resistance and interpretation of the results were carried out

according to the Clinical Recommendations «Determination of the sensitivity of microorganisms to antimicrobial agents» (version 2015), EUCAST (2015). Molecular serotyping of S. pneumoniae strains was performed by multiplex PCR (MPCR).

Results. 484 children aged 2–7 years, attending preschool institutions of the Republic of Tatarstan, were studied. The S. pneumoniae carrier status was 25.9% of children living in the city and 37.5% of children from rural areas with the associative nature of the nasopharyngeal microbiota in children with a long carrier period. Analysis of the serotype composition of pneumococci showed the dominance of «vaccine» serotypes; the appearance of «non-vaccine» serotypes; tendency to increase in the number of encapsulated pneumococci. An insignificant increase in the antibiotic resistance and incidence of polyresistant nasopharynegeal pneumococci was revealed.

The conclusion. The study of pneumococcal nasopharyngeal carriage with potential virulent properties and antibiotic resistance is a prerequisite for the development of preventive measures and antimicrobial therapy algorithms.

Key words: bacteria carrier, serotype, Streptococcus pneumonia.

(For citation: Zaripova A.Z., Bayazitova L.T., Tyupkina O.F., Chazova T.A., Tyurin Y.A., Isaeva G.Sh., Pokrovskaya E.M. Phenotypic and genotypic properties of Streptococcus pneumoniae in case of bacteria carrying. Practical Medicine. 2018)

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