E.V. MOKRETSOVA¹, G.S. TOMILKA¹, YU.G. KOVALSKIY¹, A.YU. SHCHUPAK¹, V.V. MALEEV²

 ¹Far Eastern State Medical University, Khabarovsk

²Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow

Contact details:

Mokretsova E.V. — PhD (medicine), Associate Professor of the Department of Infectious Diseases and Epidemiology

Address: 35 Muravyov-Amursky St., Khabarovsk, Russian Federation, 680000, tel.: +7-962-584-09-21, e-mail: bremer68@mail.ru

The purpose — to determine pathogenetic connections between the level of pro-inflammatory immunocytokines and oxidative stress indicators in the systemic blood circulation in patients with tick-borne rickettsiosis.

Material and methods. The levels of some interleukins and indicators of the oxidant-prooxidant system in patients with a moderate form of tick-borne rickettsiosis caused by R. heilongjiangensis were researched and analyzed.

Results. At the height of the disease, we found a statistically significant increase (p < 0.01) in the blood serum of all pro-inflammatory cytokines studied (IL-6, IL-8, TNF), combined with a significantly pronounced activation of free radical oxidation processes (p ˂ 0.0002). During the early convalescence period, there was a decrease in the level of IL-6 and TNF by 7 and 2.6 times, respectively (p < 0.0001), while the level of IL-8 statistically significantly increased twofold by the time of discharge relative to the height of the disease (p < 0.05). In the same period, oxidative stress indicators increased statistically significantly (p ˂ 0.0001) compared to the acute period: Ssp — 6 times; h — 1.3 times; Sind-1 — 1.3 times; Slum — 1.4 times; Sind-2 — 1.3 times. A correlation analysis revealed a significant direct relation between the content of the studied interleukins and chemiluminescence indices in the blood serum.

Conclusion. Changes in cytokine status identified during the study in patients with tick-borne rickettsiosis caused by R heilongjiangensis, indicate the participation of interleukins in the disease pathogenesis. The increase in the level of reactive oxygen species during early convalescence may indicate that clinical recovery does not reflect the pathogenetic completion of the infection. The revealed correlation between indicators of cytokine and oxidative status testifies to their joint participation in the pathogenesis of tick-borne rickettsiosis caused by R. heilongjiangensis.

Key words: tick-borne rickettsiosis, cytokines, oxidative stress.

(For citation: Mokretsova E.V., Tomilka G.S., Kovalskiy Yu.G., Shchupak A.Yu., Maleev V.V. Pathogenetic connections of cytokine and oxidative states of patients with tick-borne rickettsiosis caused by R. Heilongjiangensis. Practical medicine. 2023. Vol. , № , P.)

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