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  • Investigation of the possibility of using cultured autologous limbal epithelial stem cells on the collagen scaffold to treat the limbal deficiency in an experiment

    Редактор | 2017, Practical medicine 09 (17) Ophthalmology. Part 2 | 6 сентября, 2017

    A.V. BEZUSHKO¹, A.S. DUBOVIKOV¹, A.N. KULIKOV¹, S.V. CHURASHOV¹, V.F. CHERNYSH¹, M.I. BLINOVA², O.I. ALEKSANDROVA², A.A. SUETOV¹, I.O. GAVRILYUK¹

    ¹Military Medical Academy named after S.M. Kirov, 6 Akademika Lebedeva Str., Saint Petersburg, Russian Federation, 194044

    ²Institute of Cytology of the Russian Academy of Science, 4 Tikhoretskiy Ave., Saint Petersburg, Russian Federation, 194064

     Bezushko A.V. — ophthalmologist, e-mail: konkievaav@yandex.ru

    Dubovikov A.S. — сlinical resident, e-mail: dubovikovanatolyi@gmail.com

    Kulikov A.N. — Head of the Department of Ophthalmology, D. Med. Sc., e-mail: alexey.kulikov@mail.ru

    Churashov S.V. — Professor of the Department of Ophthalmology, D. Med. Sc., e-mail: churashoff@mail.ru

    Chernysh V.F. — Associate Professor of the Department of Ophthalmology, Cand. Med. Sc., e-mail: vfchernysh@gmail.com

    Blinova M.I. — Cand. Biol. Sc., senior research scientist, Laboratory of cell biology in culture, e-mail: mira.blinova@mail.ru

    Aleksandrova O.I. — junior research associate, Laboratory of cell biology in culture, e-mail: elga.aleks@gmail.com

    Suetov A.A. — ophthalmologist, Cand. Med. Sc., e-mail: ophtalm@mail.ru

    Gavrilyuk I.O. — ophthalmologist, e-mail: iluaayaks@gmail.com.

     A promising treatment method for patients with limbal stem cell deficiency (LSCD) is the transplantation of cultured limbal epithelial stem cells (LESC) obtained from minimal biopsy samples in various carriers. To investigate the possibility of using cultured autologous LESC on the collagen scaffold (CS) to eliminate LSCD in the experiment. In 30 rabbits (60 eyes) the mechanical LSCD model was created. Animals were divided into 3 groups. The 1st and the 2nd were treatment groups, the 3rd — the control group. In the 1st group collagen scaffold (CS) with cultivated LESC was placed on denuded cornea twice with the 3-days interval, in the 2nd group it was placed once. In the control group CS with no LESC was applied once. On the 90th day transparency noticeably increased in the 1st group in contradistinction to the 2nd and control groups, where vascularization and opacification were sufficiently noticeable. According to impression cytology, epithelium with no goblet cells was found in the 1st group in 90% of cases. Histological examination confirmed it. In the 2nd group the presence of goblet cells was indicated in 50% of cases, in the control group goblet cells were indicated in all the animals. Double application of CS with cultured LESC proved to be effective in LSCD treatment in the experiment and can be recommended for limited clinical trials.

    Key words: limbal epithelial stem cells, collagen scaffold, limbal deficiency.

    References

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    Метки: 2017, A.A. SUETOV, A.N. KULIKOV, A.S. DUBOVIKOV, A.V. BEZUSHKO, collagen scaffold, I.O. GAVRILYUK, limbal deficiency, limbal epithelial stem cells, M.I. BLINOVA, O.I. ALEKSANDROVA, Practical medicine 09 (17) Ophthalmology. Part 2, S.V. CHURASHOV, V.F. CHERNYSH

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