T.E. EVERSTOVA¹, T.YA. NIKOLAEVA², S.A. CHUGUNOVA²

 ¹Republic Hospital No. 2 — Center for Emergency Medical Aid, Yakutsk

²North-Eastern Federal University named after M.K. Ammosov, Yakutsk

Contact details:

Everstova T.E. — post-graduate student of the Department of Neurology and Psychiatry

Address: 58 Belinsky St., Yakutsk, Russian Federation, 677000, tel.: +7-914-227-68-19, e-mail: selectir_66@mail.ru

 The purpose — to study the association of rs2317676 polymorphic variants of the GPIIIa gene with the development of aspirin resistance in patients with atherothrombotic variant of ischemic stroke.

Material and methods. The article presents data of 100 patients with acute ischemic stroke and 100 people from the control group. All the patients received aspirin at a dose of 300 mg per day during the first day of the disease and 100 or 125 mg per day thereafter. The control group received aspirin as a primary prophylaxis for cardiovascular diseases. The study measured platelet aggregation on day 5–7 of taking the drug. The rs2317676 polymorphism of the GPIIIa gene was considered as genetic marker.

Results. The frequency of A/G polymorphism rs2317676 of the GPIIIa gene was lower among individuals with low platelet aggregation compared to individuals with high platelet aggregation (p = 0.023; OR = 0.33; 95% CI: 0.13–0.89).

Conclusion. Carriage of the A/G genotype rs2317676 polymorphism of the GPIIIa gene influences an increase in platelet aggregation and the development of aspirin resistance.

Key words: ischemic stroke, aspirin, aspirin resistance, genetic polymorphisms.

REFERENCES

1. Shuteeva E.Yu. Analysis of clinical and epidemiological indicators of ischemic stroke. Regional’nyy vestnik, 2020, no. 2 (41), pp. 16–17 (in Russ.).
2. Espinosa E.V., Murad P.J., Khasawneh F.T. Aspirin: Pharmacology and Clinical Applications. Thrombosis, 2011, vol. 2012, pp. 1–15.
3. Sehgal M., Wood S.K., Ouslander J.G.et al. Aspirin in Older Adults: Need for Wider Utilization in Secondary Prevention and Individual Clinical Judgments in Primary Prevention. J Cardiovasc Pharmacol Ther, 2017, vol. 22 (6), pp. 511–513.
4. Yi X., Lin J., Wang C. et al. A comparative study of dual versus monoantiplatelet therapy in patients with acute large-artery atherosclerosis stroke. Journal of Stroke and Cerebrovascular Diseases, 2014, vol. 23, pp. 1975–1981.
5. Bugnicourt J.M., Roussel B., Garcia P.Y. et al. Aspirin non-responder status and early neurological deterioration: a prospective. Clinical Neurology and Neurosurgery, 2011, vol. 113, pp. 196–201.
6. Kruchinina M.V., Gromov A.A., Rabko A.V. et al. Aspirin resistance as a risk marker for venous thromboembolic complications. Ateroskleroz, 2018, vol. 14, no. 4, pp. 40–55 (in Russ.).
7. Guirgis M., Thompson P., Jansen S. Review of aspirin and clopidogrel resistance in peripheral arterial disease. J Vasc Surg, 2017, vol. 66 (5), pp. 1576–1586.
8. Grinshteyn Yu.I., Kosinova P.A., Grinshteyn I.Yu. Candidate genes for resistance to acetylsalicylic acid and their relationship with the risk of developing cardiovascular accidents. Kardiovaskulyarnaya terapiya i profilaktika, 2013, vol. 12, no. 1, pp. 67–72 (in Russ.).
9. Hayderkareemkataa AL-A., EhabD.S., Safa A F. et al. The ITGB3 Genevariant among Sample of Glanzmann thrombasthenia Iraqi Patients. Medico-legal Update, 2021, vol. 21 (1), pp. 333–339.
10. Yi X., Han Z., Zhou Q. et al. Interactions among COX-2, GPIIIa and P2Y1 variants are associated with aspirin responsiveness and adverse events in patients with ischemic stroke. Ther Adv Neurol Disord, 2017, vol. 10 (3), rr. 161–170.
11. Yi X., Lin J., Wang Y. et al. Interaction among CYP2C8, GPIIIa and P2Y12 variants increase susceptibility to ischemic stroke in Chinese population. Oncotarget, 2017, vol. 8 (41), rr. 70811–70820.
12. Yi X., Zhou Q., Wang C. et al. Platelet receptor Gene (P2Y12, P2Y1) and platelet glycoprotein Gene (GPIIIa) polymorphisms are associated with antiplatelet drug responsiveness and clinical outcomes after acute minor ischemic stroke. Eur J Clin Pharmacol, 2017, vol. 73 (4), rr. 437–443.
13. Yi X., Lin J., Zhou Q. et al. The TXA2R rs1131882, P2Y1 rs1371097 and GPIIIa rs2317676 three-loci interactions may increase the risk of carotid stenosis in patients with ischemic stroke. BMC Neurol, 2019, vol. 19 (1), p. 44.