L.А. МUSINA, R.F. SHAKIROV, R.Z. КADIROV, О.R. SHANGINA

All-Russian Center for Eye and Plastic Surgery of the Ministry of Health of the Russian Federation, 67/1 R. Zorge Str., Ufa, Russian Federation, 450075

  Musina L.A. — Doc. Biol. Sc., Leading Researcher of the Morphology Department, tel. (347) 293-42-35, e-mail: morphoplant@mail.ru

Shakirov R.F. — physician-ophthalmosurgeon, tel. (347) 293-42-28, e-mail: rust.ufa@mail.ru.

Kadyrov R.Z. — D. Sc. (medicine), Academic secretary, Acting Director General, tel. (347) 293-42-35, e-mail: morphoplant@mail.ru

Shangina O.R. — Doc. Biol. Sc., Leading Researcher, Head of the Tssue Conservation Laboratory, tel. (347) 293-42-35, e-mail: alloolga@mail.ru

The histological, immunohistochemical and electron-microscopic methods were used to investigate the enucleated eyeballs of the experimental rabbits following the chemical burn by the alkali (2.5% of sodium hydroxide solution) and perilimbal insertion of the allogeneic biomaterial. The dispersed form of “regeneration stimulator” biomaterial was used for the operation. This type of the biomaterial is produced in the All-Russian Eye and Plastic Surgery Centre under the brand name “Alloplant”. The aim of the investigation was to reveal the impact of the perilimbal inserted allogeneic biomaterial upon the damaged cornea of the rabbit eyes following the chemical burn. The morphological investigations of the cornea and zone of the eye limus were carried out on the 7^th, 14^th, 30^th, 90^th and 180^th days after the operation. It was established that the perilimbal biomaterial insertion had stimulated regenerative processes in the pathologically changed cornea. Due to the low degree of the expression by the cytokine cells which was transforming the growth factor TGF-β1 (fibrous factor), the inhibition of the rough tissue scarring process was taking place, which contributed to the full restoration of the corneal structural elements.

Key words: allogeneic biomaterial, perilimbal insertion, corneal regeneration, transforming growth factor TGF-β

(For citation: Musina L.A., Shakirov R.F., Kadyrov R.Z., Shangina O.R. Experimental and morphological investigation of the dispersed allogeneic biomaterial effect on corneal regeneration. Practical Medicine. 2018)

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