R.T. NIGMATULLIN, R.Z. KUTUSHEV, B.R. MOTYGULLIN

All-Russian Eye and Plastic Surgery Center, 67/1 Zorge Str., Ufa, Russian Federation, 420075

Nigmatullin R.T. — D. Med. Sc., Deputy Director General for Scientific Work, tel. (347) 224-68-03, e-mail: nigmatullinr@mail.ru

Kutushev R.Z. — Head of the Ophthalmology Department №3, tel. (347) 224-68-19, e-mail: rinat-dok@yandex.ru

Motygullin B.R. — probationer-researcher, tel. (347) 224-68-19, e-mail: mbr24@yandex.ru

 The publication contains the results of experimental transplantation of the structured elastin biomaterial (patent No.2440148) into the Wistar rat eyeball osseous defect of 7 mm in diameter (experimental series). In the control series (n=23) the similar osseous defect remained intact. The histological studies of the graft and tissue bed were carried out on the 30, 90 and 360 days following the experiment. The studies allowed to reveal the following types of osteogenesis against the background of the biomaterial transplantation: oppositional growth of the osseous wall in the perifocal zone, marginal regeneration in the contact zone of the transplant with the osseous bed, endesmal ossification in the boundary loose connective tissue. Besides, there were for the first time revealed the intraelastin loci of osteogenesis directly in the elastin transplant. The obtained results make it possible to recommend elastin biomaterials for osteoplastic operations in cranio-facial surgery.

Key words: elastin biomaterials, reparative osteogenesis, reconstructive surgery of the orbit.

REFERENCES

1. Anabami N., Mithieux S.M., Camci-Unal G., Dokmeci M.R., Weiss A.S., Khademhosseini A. Elastomeric recombinant protein-based biomaterials. Biochemical Engineering Journal, 2013, vol. 77, rr. 110-118.
2. Derricks K.E., Rich C.B., Buczek-Thomas J.A., Nugent M.A. Ascorbate enhances elastin synthesis in 3D tissue-engineered pulmonary fibroblasts constructs. Tissue and Cell, 2013, vol. 45, rr. 253-260.
3. Nigmatullin R.T., Kutushev R.Z. Application of elastin biomaterial in reconstructive surgery of orbit walls. Prakticheskaya meditsina, 2016, no. 6 (98), pp. 119-121 (in Russ.).
4. Regenerativnaya meditsina. Biomaterial Alloplant v oftal’mokhirurgii, pod obshch. red. E.R. Muldasheva [Regenerative medicine. Biomaterial Alloplant in ophthalmic surgery / under the general. Ed. by E.R. Muldashev]. Ufa: GUP “Bashkortostan”, 2014. 432 p.
5. Muldashev E.R., Nigmatullin R.T., Galimova V.U. et al. Ksenogennyy biomaterial dlya regenerativnoy khirurgii: patent no. 2440148, prioritet 21.12.2009, opublik. 20.01.2012 [Xenogenic biomaterial for regenerative surgery: patent No. 2440148, priority 21.12.2009, published. 20.01.2012], byul. no. 2.
6. Omel’yanenko N.P., Slutskiy L.I. Soedinitel’naya tkan’ (gistofiziologiya i biokhimiya). V 2 t. T. II, pod red. akademika RAN i RAMN S.P. Mironova [Connective tissue (histophysiology and biochemistry). In 2 volumes. Volume II. Ed. by academician of RAS and RAMS S.P. Mironov]. Moscow: Izvestiya, 2010. 600 p.