A.V. LUKSHA, N.A. MAXIMOVICH, V.V. HARCHAKOVA, I.E. GULYAI, V.R. SHULIKA

 Grodno State Medical University, Grodno, Republic of Belarus

  Contact details:

Luksha A.V. — Assistant Lecturer of the Department of Pediatrics I

Address: 80 Gorkogo St., Grodno, Republic of Belarus, 230009, tel.: +375 (0152) 44-81-21, e-mail: drluksha@mail.ru

 The purpose — to study the effect of the polymorphic variants 894G/T and T786C of NOS3 gene of the endothelial nitric oxide synthase gene on the levels of their expression products — eNOS and NO in children with arterial hypertension.

Material and methods. We examined 111 children, including 51 patients with arterial hypertension, 30 patients with high-normal blood pressure and 30 healthy children. The polymorphism of the study genes was studied using a real-time polymerase chain reaction. Determination of plasma concentrations of eNOS was performed with enzyme immunoassay. NO metabolites, nitrateandnitrite (NOx) production were determined using the Griess reaction.

Results. Among the children of the Grodno region, the homozygous GG genotype and the G-allele of the polymorphic locus 894G/T of the NOS3 gene and the heterozygous TC genotype and the T-allele of the T786C polymorphic variant of the NOS3 gene predominate. The presence of the T allele 894G/T of the NOS3 gene in children with arterial hypertension is associated with a decrease eNOS expression and NOx levels compared with T allele carriers among healthy children. Heterozygous genotype 786TC and allele C of T786C polymorphism of the NOS3 gene in children with arterial hypertension are characterized by a low level of NOx compared with healthy children.

Key words: children, arterial hypertension, endothelial nitric oxide synthase, nitric oxide metabolites, NOS3 gene polymorphism.

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