Clinical and morphological aspects of the direct gene therapy impact by Neovaskulgen in patients with peripheral arterial occlusive disease
M.V. PLOTNIKOV1,2, M.O. MAVLIKEEV3, I.I. SHAMSUTDINOVA1
1Republican Clinical Hospital of the MH of RT, 138 Orenburgsky tract, Kazan, Russian Federation, 420064
2Kazan State Medical Academy, 36 Butlerov St., Kazan, Russian Federation, 420012
3Kazan (Volga region) Federal University, 18 Kremlevskaya St., Kazan, Russian Federation, 420008
Plotnikov M.V. — Surgery of the Department of Vascular Surgery № 1, Assistant of the Department of cardiology, endovascular and cardiovascular surgery, tel. +7-917-265-19-03, e-mail: piotnikov_mv@bk.ru
Mavlikeev M.O. — Junior Researcher, tel. +7-917-265-19-03, e-mail: MOMavlikeev@kpfu.ru
Shamsutdinova I.I. — doctor of functional diagnostics, tel. +7-927-670-04-55, e-mail: ilsgum@rambler.ru
Clinical and morphological data of «Neovaskulgen» therapy in patients with peripheral arterial disease were presented. Twice repeated intramuscular injections of Neovaskulgen (according to the manufacturer’s instructions) were performed to 10 patients with ischemia IIb-III grade by Pokrovsky-Fontaine. Standart tredmill test, ankle-brachial index estimation were performed. Injured muscle biopsies taken before and 3 months after injection was histologically studied. Pain-free walking distance increase to 31.74% at 3 months and no limits to walk at six months were detected. There were ankle-brachial index increase to 64.7% at 6 months. In intact muscles therapy with Neovaskulgen leads to increase of mean cross-sectional muscle fiber area without significant angiogenic effect. In muscles with decreased capillary density this therapy leads to blood supply improvement promoting regeneration of muscles by myosatellites proliferation and increase of mean cross-sectional muscle fiber area and connective tissue degradation.
Key words: peripheral arterial disease, gene therapy, Neovaskulgen, regeneration, muscle biopsy, immunohistochemistry.
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