M.V. PLOTNIKOV^1,2, M.O. MAVLIKEEV³, I.I. SHAMSUTDINOVA¹

¹Republican Clinical Hospital of the MH of RT, 138 Orenburgsky tract, Kazan, Russian Federation, 420064

²Kazan State Medical Academy, 36 Butlerov St., Kazan, Russian Federation, 420012

³Kazan (Volga region) Federal University, 18 Kremlevskaya St., Kazan, Russian Federation, 420008

Plotnikov M.V. — Surgery of the Department of Vascular Surgery № 1, Assistant of the Department of cardiology, endovascular and cardiovascular surgery, tel. +7-917-265-19-03, e-mail: [email protected]

Mavlikeev M.O. — Junior Researcher, tel. +7-917-265-19-03, e-mail: [email protected]

Shamsutdinova I.I. — doctor of functional diagnostics, tel. +7-927-670-04-55, e-mail: [email protected]

Clinical and morphological data of «Neovaskulgen» therapy in patients with peripheral arterial disease were presented. Twice repeated intramuscular injections of Neovaskulgen (according to the manufacturer’s instructions) were performed to 10 patients with ischemia IIb-III grade by Pokrovsky-Fontaine. Standart tredmill test, ankle-brachial index estimation were performed. Injured muscle biopsies taken before and 3 months after injection was histologically studied. Pain-free walking distance increase to 31.74% at 3 months and no limits to walk at six months were detected. There were ankle-brachial index increase to 64.7% at 6 months. In intact muscles therapy with Neovaskulgen leads to increase of mean cross-sectional muscle fiber area without significant angiogenic effect. In muscles with decreased capillary density this therapy leads to blood supply improvement promoting regeneration of muscles by myosatellites proliferation and increase of mean cross-sectional muscle fiber area and connective tissue degradation.

Key words: peripheral arterial disease, gene therapy, Neovaskulgen, regeneration, muscle biopsy, immunohistochemistry.

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