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  • Basophil test in practical medicine

    Редактор | 2019, Practical medicine part 17 №1. 2019 | 17 января, 2019

    V.V. LABIS1, E.A. BAZIKYAN1, S.V. SIZOVA3, V.V. ZHELEZNY6, N.V. BYCHKOVA4, 5, I.G. KOZLOV2

     1Moscow State Medical and Dental University named after A.I. Evdokimov

    2Federal Scientific and Clinical Center “Pediatric Hematology, Oncology and Immunology named after D. Rogachev” of the Ministry of Health of the Russian Federation, Moscow, Russian Federation

    3 Institute of Bioorganic Chemistry named after Academician M.M. Shemyakin and Yu.A. Ovchinnikov of the Russian Academy of Sciences, Moscow, Russian Federation

    4 All-Russian Center for Emergency and Radiation Medicine named after A.M. Nikiforov of the EMERCOM of Russia, Saint Petersburg, Russian Federation

    5 First St. Petersburg State Medical University named after I.P. Pavlov, Saint Petersburg, Russian Federation

    6 Saint-Dent Clinic LLC, Moscow, Russian Federation

      Contact details:

    Labis V.V. – Ph. D. (medicine), Assistant of the Department of Oral Surgery

    Address: 20 Delegatskaya St., building 1, Moscow, Russian Federation, 127473, tel: +7-962-977-77-72, e-mail: varvara2001@mail.ru

    The objective of the present study was to study the immunological reactions, in particular, the activation of basophils of the venous blood of patients to the «bioinert» medical alloys TiO2. Before planned surgical interventions, certified medical devices in a laminar box were immersed in sterile double distillate tubes, exposed to 35 kHz ultrasound, simulating the functioning in the human body, according to the previously developed technique described in patent No. 2611013. The resulting supernatants were added to a sample of venous blood of patients to conduct a modified basophil test using the «Allergenicity Kit, Cellular Analysis of Allergy» test system. Using flow cytofluorimetry, basophil activation or lack thereof was assessed. Based on the basophil activation index in the sample under study, less than 1 or equal to 1, this medical object was recommended for use.

     Key words: basophil test, metal alloys based on titanium dioxide (TiO2), biotolerance, allergy to metals.

    REFERENCES

    1. Labis V.V. New view on the bioinert of dental implants. Meditsinskaya immunologiya, 2011, vol. 13, no. 4–5, pp. 299–550 (in Russ.).
    2. Labis V.V, Sizova S.V., Khaydukov S.V., Bazikyan E.A., Kozlov I.G. The role of metal nanoparticles in the mechanism of osseointegration of dental implants. Rossiyskiy immunologicheskiy zhurnal, 2012, vol. 9 (18), no. 2 (2), pp. 48–53 (in Russ.).
    3. Thomas P.1, Iglhaut G., Wollenberg A. et al. Allergy or tolerance: reduced inflammatory cytokine response and concomitant IL-10 production of lymphocytes and monocytes in symptom-free titanium dental implant patients. Biomed Res Int., 2013. DOI: 10.1155/2013/539834. E-pub., 2013, Sep. 11.
    4. De Amici M., Barocci F., Caimmi S., Nespoli L., Licari A., Giuliani G., Marseglia G. Clinical use of Basophil Activation Test (BAT) in drug, food and hymenoptera venom allergies. Minerva Pediatr, 2018, Oct 4. DOI: 10.23736/S0026-4946.18.05144-7. [E-pub ahead of print].
    5. Mayumi S., Akemi O., Keigo Y., Hiroki M., Hiroki I., Noriko N., Shoji K. Manifestation of intraoperative anaphylactic shock along with latex allergy: a pediatric case report. J Med Invest, 2018, no. 65 (3–4), pp. 292–295. DOI: 10.2152/jmi.65.292.
    6. Mombelli A., Hashim D., Cionca N. What is the impact of titanium particles and biocorrosion on implant survival and complications? A critical review. Clin Oral Implants Res, 2018, Oct. 29, suppl. 18, pp. 37–53. DOI: 10.1111/clr.13305.

    Метки: 2019, allergy to metals, basophil test, biotolerance, E.A. BAZIKYAN, I.G. KOZLOV, metal alloys based on titanium dioxide (TiO2), N.V. BYCHKOVA, Practical medicine part 17 №1. 2019, S.V. SIZOVA, V.V. LABIS, V.V. ZHELEZNY

    ‹  Effect of structural and functional properties of demineralized bone matrix on the possibility of settling cells and their viability to create effective tissue engineering constructions  Bone repair in orthotopic allo- and xeno-transplantation ›
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