V.V. LABIS¹, E.A. BAZIKYAN¹, S.V. SIZOVA³, V.V. ZHELEZNY⁶, N.V. BYCHKOVA^4, 5, I.G. KOZLOV²

 ¹Moscow State Medical and Dental University named after A.I. Evdokimov

²Federal Scientific and Clinical Center “Pediatric Hematology, Oncology and Immunology named after D. Rogachev” of the Ministry of Health of the Russian Federation, Moscow, Russian Federation

³ Institute of Bioorganic Chemistry named after Academician M.M. Shemyakin and Yu.A. Ovchinnikov of the Russian Academy of Sciences, Moscow, Russian Federation

⁴ All-Russian Center for Emergency and Radiation Medicine named after A.M. Nikiforov of the EMERCOM of Russia, Saint Petersburg, Russian Federation

⁵ First St. Petersburg State Medical University named after I.P. Pavlov, Saint Petersburg, Russian Federation

⁶ Saint-Dent Clinic LLC, Moscow, Russian Federation

  Contact details:

Labis V.V. – Ph. D. (medicine), Assistant of the Department of Oral Surgery

Address: 20 Delegatskaya St., building 1, Moscow, Russian Federation, 127473, tel: +7-962-977-77-72, e-mail: [email protected]

The objective of the present study was to study the immunological reactions, in particular, the activation of basophils of the venous blood of patients to the «bioinert» medical alloys TiO2. Before planned surgical interventions, certified medical devices in a laminar box were immersed in sterile double distillate tubes, exposed to 35 kHz ultrasound, simulating the functioning in the human body, according to the previously developed technique described in patent No. 2611013. The resulting supernatants were added to a sample of venous blood of patients to conduct a modified basophil test using the «Allergenicity Kit, Cellular Analysis of Allergy» test system. Using flow cytofluorimetry, basophil activation or lack thereof was assessed. Based on the basophil activation index in the sample under study, less than 1 or equal to 1, this medical object was recommended for use.

 Key words: basophil test, metal alloys based on titanium dioxide (TiO₂), biotolerance, allergy to metals.

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